Calculate Chloropyll Content

The limit is 99 samples
Number can be adjusted for individual samples later
Experimental Procedure

According to Porra et al. use a final concentration of 80 % aceton to extract the chlorophyll from your sample. Centrifuge, to remove any cell debris and measure the absorbance of the supernatant at 646 nm, 663 nm and 750 nm.

Based on:

Porra, R.J., Thompson, W.A., and Kriedemann, P.E. (1989) Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents; verification of the concentration of chlorophyll standards by atomic absorption spectroscopy. Biochimica et Biophysica Acta, 975, 384-394